Laboratory of Biochemistry
Laboratory of Biochemistry
Japanese
Cell
Welcome to the website of the Laboratory of Biochemistry, School of Pharmacy, Kitasato University. Our laboratory is studying the molecular mechanisms of gene expression and signal transduction pathways. Gene expression program is tightly regulated by various extracellular stimuli and its misregulation causes human diseases such as cancer. We use various biochemical and cell biological techniques to tackle the mysteries of biology. If you are interested in the lab, feel free tocontact us!.
Research Achievements
The oncoprotein DEK controls growth-regulated gene expression by enhancing the DNA-binding activity of basic leucine zipper transcription factors
FEBS J. 2025 May 2. doi: 10.1111/febs.70124. Online ahead of print.
https://pubmed.ncbi.nlm.nih.gov/40318137/Function of HP1BP3 as a linker histone is regulated by linker histone chaperones, NPM1 and TAF-I
Miharu Hisaoka, Tetsuro Komatsu, Takuma Hashimoto, Jianhuang Lin, Yasuyuki Ohkawa, Mitsuru Okuwaki
Epigenetics Chromatin 2025 Mar 27;18(1):14.
https://pubmed.ncbi.nlm.nih.gov/40140990/
Nuclear reorganization by NPM1-mediated phase separation triggered by adenovirus core protein VII
Genoveso MJ, Okuwaki M, Kato K, Nagata K, Kawaguchi A.
Microbiology Spectrum (2024)Aug20:e0041624
DOI: 10.1128/spectrum.00416-24
Measurement of the intracellular active metabolites of thiopurine drugs to evaluate the enzymatic activity of nudix hydrolase 15 in human blood samples.
Okamoto H, Tanaka Y, Shibagaki Y, Kuronuma S, Miyatani Y, Umeda S, Mishiro-Sato E, Takeuchi O, Hattori S, Kobayashi T, Okuwaki M.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Feb 15;1234:123993. doi:10.1016/j.jchromb.2024.123993. Epub 2024 Jan 18.
A Proteomic Analysis of Detergent-Resistant Membranes in HIV Virological Synapse: The Involvement of Vimentin in CD4 Polarization.
Iida N, Kawahara M, Hirota R, Shibagaki Y, Hattori S, Morikawa Y.
Viruses. 2023 May 28;15(6):1266. doi: 10.3390/v15061266.
The stability of NPM1 oligomers regulated by acidic disordered regions controls the quality of liquid droplets.
Okuwaki M, Ozawa SI, Ebine S, Juichi M, Umeki T, Niioka K, Kikuchi T, Tanaka N.J Biochem. 2023 Oct 31;174(5):461-476. doi: 10.1093/jb/mvad061.
https://pubmed.ncbi.nlm.nih.gov/37540843/Protective function of the SQSTM1/p62-NEDD4 complex against methylmercury toxicity.
Takanezawa Y, Harada R, Shibagaki Y, Kashiwano Y, Nakamura R, Ohshiro Y, Uraguchi S, Kiyono M..
Biochem Biophys Res Commun., 18 (609) :134-140 (2022)
https://pubmed.ncbi.nlm.nih.gov/35452957/
Silencing of SmgGDS, a Novel mTORC1 Inducer That Binds to RHEBs, Inhibits Malignant Mesothelioma Cell Proliferation
Sato T, Mukai S, Ikeda H, Mishiro-Sato E, Akao K, Kobayashi T, Hino O, Shimono W,?Shibagaki Y, Hattori S, Sekido Y..
Mol Cancer Res, 19 (5) :921-931 (2021)
https://pubmed.ncbi.nlm.nih.gov/33574130/
SET-NUP214 and MLL cooperatively regulate the promoter activity of the HoxA10 gene
Cigdem S, Saito S, Nishikata D, Nagata K,?Okuwaki M..
Genes Cells, 26 (10) :830-837 (2021)
https://pubmed.ncbi.nlm.nih.gov/34320268/
G-patch domain-containing protein 4 localizes to both the nucleoli and Cajal bodies and regulates cell growth and nucleolar structure
Hirawake-Mogi H, Thanh Nhan NT,?Okuwaki M..
Biochem Biophys Res Commun., 25 (559) :99-105 (2021)
https://pubmed.ncbi.nlm.nih.gov/33933995/