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Research Center for Anti-infectious Drugs

  • Research
  • Members
  • Publications

Research Synopsis

     The emergence and dissemination of antibiotic-resistant pathogens in the communities and hospital environments represents a serious challenge against infection control and antimicrobial chemotherapy. This group of investigators are interested in the emergence and spread of antibiotic-resistant pathogens, particularly methicillin-resistant Staphylococcus aureus (MRSA). Currently, this laboratory is mainly engaged in the following three projects: studying on the mechanisms of antibiotic resistance in Staphylococcus aureus; development of simple and rapid diagnostic kits for infectious diseases ; and serving as a central laboratory for nationwide surveillance programs on antibiotic resistance. In addition, collaborations are on progress with in- and out-investigators for search and development of new antimicrobial agents.

Current projects include:

  1. Having been designated as the central laboratory of the three Academic Societies Joint Antimicrobial Susceptibility Surveillance Program (as the base of Japan’s first public surveillance), we have been conducting surveillance on behalf of the Japanese Society of Chemotherapy (JSC), Japanese Association for Infectious Diseases (JAID), and Japanese Society for Clinical Microbiology (JSCM) since 2006.
  2. Studies on the mechanisms of antibiotic resistance in pathogen bacteria, particularly for the methicillin resistant S. aureus (MRSA), β-lactam antibiotic-induced vancomycin resistant S. aureus (BIVR), vancomycin intermediate S. aureus (VISA), and heterogeneous VISA (hVISA), and daptomycin non-susceptible S. aureus and linezolid resistant S. aureus.
  3. Studies on treatments of bacterial infections including combination therapies for various multidrug-resistant bacteria including MDRP, MDAG, and carbapenemase-producing strains.
  4. Survey on drug susceptibility of chlamydia in Japan.
  5. Development of immunochromatographic methods for rapid diagnosis of infectious diseases.
  6. Cooperation with Kitasato University Hospital and Kitasato Institute Hospital for the infection control programs.
  7. Participation in the preparation of the MRSA treatment guidelines as a joint project of the JSC and JAID.
  8. Evaluation of products under development, post-marketing surveys, and chlorine dioxide assessments as commissioned research.
  9. Drug susceptibility analysis and epidemiological surveys of fungi,　particularly candida.
  10. Development of novel anti-infectious drugs (collaborative research).
Researchers
Growing MRSA in VCM 4μg/ml
  • Research Center for Anti-infectious Drugs

Staff

  • Hanaki Hideaki
    Project Professor (Director)
  • Cui Longzhu
    Assistant Director

2012

  1. Tsutsui A, Hirose T, Ishiyama A, Iwatsuki M, Yokota A, Maruyama H, Matsui H, Otoguro K, Hanaki H, Omura S, Sunazuka T. : Antimalarial C-9 oxime derivatives from desmycosin, produced by click chemistry.　 J Antibiot (Tokyo). 2012 12. doi: 10.1038/ja.2012.113. [Epub ahead of print].
  2. Matsumoto T, Hanaki H, Kimura T, Nemoto M, Higashihara M, Yokota H, Oda S, Akiyama N, Miyao N, Yoshida M, Yukioka T, Soma K, Ohyashiki K, Suzuki Y, Arai T, Ikegami K, Ichiwata T, Otsuka Y, Kobayashi M, Totsuka K, Sunakawa K. ; ABK Dose Finding Study Group.　 Clinical efficacy and safety of arbekacin sulfate in patients with MRSA sepsis or pneumonia: a multi-institutional study.　 J Infect Chemother. 2012 Dec 22. [Epub ahead of print].
  3. Matsumoto T, Hanaki H, Kimura T, Nemoto M, Higashihara M, Yokota H, Oda S, Akiyama N, Miyao N, Yoshida M, Yukioka T, Soma K, Ohyashiki K, Suzuki Y, Arai T, Ikegami K, Ichiwata T, Otsuka Y, Kobayashi M, Totsuka K, Sunakawa K. : ABK Dose Finding Study Group. Clinical efficacy and safety of arbekacin sulfate in patients with MRSA sepsis or pneumonia: a multi-institutional study. J Infect Chemother. 2012 Dec 22. [Epub ahead of print].
  4. Tsutsui A, Hirose T, Ishiyama A, Iwatsuki M, Yokota A, Maruyama H, Matsui H, Otoguro K, Hanaki H, Omura S, Sunazuka T. : Antimalarial C-9 oxime derivatives from desmycosin, produced by click chemistry. J Antibiot (Tokyo). 2012 Dec 12. doi: 10.1038/ja.2012.113. [Epub ahead of print].
  5. Takesue Y, Watanabe A, Hanaki H, Kusachi S, Matsumoto T, Iwamoto A, Totsuka K, Sunakawa K, Yagisawa M, Sato J, Oguri T, Nakanishi K, Sumiyama Y, Kitagawa Y, Wakabayashi G, Koyama I, Yanaga K, Konishi T, Fukushima R, Seki S, Imai S, Shintani T, Tsukada H, Tsukada K, Omura K, Mikamo H, Takeyama H, Kusunoki M, Kubo S, Shimizu J, Hirai T, Ohge H, Kadowaki A, Okamoto K, Yanagihara K. : Nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (SSI) in Japan. J Infect Chemother. 2012 Dec;18(6):816-26.
  6. Suga T, Ishii T, Iwatsuki M, Yamamoto T, Nonaka K, Masuma R, Matsui H, Hanaki H, Omura S, Shiomi K. : Aranorosin circumvents arbekacin-resistance in MRSA by inhibiting the bifunctional enzyme AAC(6')/APH(2″). J Antibiot (Tokyo). 2012 Oct;65(10):527-9.
  7. Yuriko Hirao, Yurika Ikeda-Dantsuji, Hidehito Matsui, Masaki Yoshida, Seiji Hori, Keisuke Sunakawa , Taiji Nakae, and Hideaki Hanaki. Low level ß-lactamase production in methicillin-resistant Staphylococcus aureus with ß-lactam-antibiotic-induced vancomycin resistance. BMC Microbiol. 2012 May 8;12(1):69.
  8. Watanabe A, Yanagihara K, Matsumoto T, Kohno S, Aoki N, Oguri T, Sato J, Muratani T, Yagisawa M, Ogasawara K, Koashi N, Kozuki T, Komoto A, Takahashi Y, Tsuji T, Terada M, Nakanishi K, Hattori R, Hirako Y, Maruo A, Minamitani S, Morita K, Wakamura T, Sunakawa K, Hanaki H, Ohsaki Y, Honda Y, Sasaoka S, Takeda H, Ikeda H, Sugai A, Miki M, Nakanowatari S, Takahashi H, Utagawa M, Kobayashi N, Takasaki J, Konosaki H, Aoki Y, Shoji M, Goto H, Saraya T, Kurai D, Okazaki M, Kobayashi Y, Katono Y, Kawana A, Saionji K, Miyazawa N, Sato Y, Watanuki Y, Kudo M, Ehara S, Tsukada H, Imai Y, Watabe N, Aso S, Honma Y, Mikamo H, Yamagishi Y, Takesue Y, Wada Y, Nakamura T, Mitsuno N, Mikasa K, Kasahara K, Uno K, Sano R, Miyashita N, Kurokawa Y, Takaya M, Kuwabara M, Watanabe Y, Doi M, Shimizu S, Negayama K, Kadota J, Hiramatsu K, Morinaga Y, Honda J, Fujita M, Iwata S, Iwamoto A, Ezaki T, Onodera S, Kusachi S, Tateda K, Tanaka M, Totsuka K, Niki Y, Matsumoto T. : Nationwide surveillance of bacterial respiratory pathogens conducted by the Surveillance Committee of Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Clinical Microbiology in 2009: general view of the pathogens' antibacterial susceptibility. J Infect Chemother. 2012 Oct;18(5):609-20.
  9. Yurika Ikeda-Dantsuji, Taiji Nakae, Koichi Ariyoshi, Hidekazu Mizuno, Hidehiko Moriyama, Osanori Nagura, Akira Suwabe, Kunihiko Fukuchi, Junichi Honda, Mitsuo Kaku, Shigeru Kohno, Hiroshige Mikamo, Yoshihito Niki, Yoshio Takesue, Kazunori Tomono, Katsunori Yanagihara, and Hideaki Hanaki. : Limited detectability of linezolid-resistant Staphylococcus aureus by the Etest method and its improvement using enriched media. J Med Microbiol. 2012 Jul;61(Pt 7):998-1002.
  10. Nihonyanagi S, Adachi Y, Onuki T, Nakazaki N, Hirata Y, Fujiki K, Takayama Y, Kanoh Y, Bandoh Y, Dantsuji Y, Hanaki H, Sunakawa K. : Emergence of linezolid-resistant Enterococcus faecalis strains from two inpatients in a pediatric ward. Kansenshogaku Zasshi. 2012 Sep;86(5):555-62. Japanese.
  11. Longzhu Cui, Hui-min Neoh, Akira Iwamoto, and Keiichi Hiramatsu. : Coordinated phenotype switching with large-scale chromosome flip-flop inversion observed in bacteria. PNAS. 2012 June 19;109(25): E1647-1656.

2011

  1. Masato Iwatsuki, Toshiya Ishimori, Tsuyoshi Yamamoto, Keiko Takata, Mihoko Mori, Kenichi Nonaka, Rokuro Masuma, Yoichi Hayakawa, Hideaki Hanaki, Kazuro Shiomi, Satoshi Omura : a. Biverlactones AeD, new circumventors of arbekacin resistance in MRSA, produced by Penicillium sp. FKI-4429. Tetrahedron. 2011, 67:6644-6648.
  2. Niki Y, Hanaki H, Matsumoto T, Yagisawa M, Kohno S, Aoki N, Watanabe A, Sato J, Hattori R, Koashi N, Terada M, Kozuki T, Maruo A, Morita K, Ogasawara K, Takahashi Y, Matsuda K, Nakanishi K, Sunakawa K, Takeuchi K, Fujimura S, Takeda H, Ikeda H, Sato N, Niitsuma K, Saito M, Koshiba S, Kaneko M, Miki M, Nakanowatari S, Takahashi H, Utagawa M, Nishiya H, Kawakami S, Aoki Y, Chonabayashi N, Sugiura H, Ichioka M, Goto H, Kurai D, Saraya T, Okazaki M, Yoshida K, Yoshida T, Tsukada H, Imai Y, Honma Y, Yamamoto T, Kawai A, Mikamo H, Takesue Y, Wada Y, Miyara T, Toda H, Mitsuno N, Fujikawa Y, Nakajima H, Kubo S, Ohta Y, Mikasa K, Kasahara K, Koizumi A, Sano R, Yagi S, Takaya M, Kurokawa Y, Kusano N, Mihara E, Nose M, Kuwabara M, Fujiue Y, Ishimaru T, Matsubara N, Kawasaki Y, Tokuyasu H, Masui K, Kido M, Ota T, Honda J, Kadota J, Hiramatsu K, Aoki Y, Nagasawa Z, Yanagihara K, Fujita J, Tateyama M, Totsuka K. : Nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese Society of Chemotherapy in 2008: general view of the pathogens' antibacterial susceptibility. J Infect Chemother. 2011 Mar 17(4):510-23.
  3. Ikeda-Dantsuji Y, Hanaki H, Nakae T, Takesue Y, Tomono K, Honda J, Yanagihara K, Mikamo H, Fukuchi K, Kaku M, Kohno S, Niki Y. : Emergence of linezolid-resistant mutants in the susceptible cell population of methicillin-resistant Staphylococcus aureus.   Antimicrob Agents Chemother. 2011 May,55(5):2466-8.
  4. Ishikawa K, Matsumoto T, Yasuda M, Uehara S, Muratani T, Yagisawa M, Sato J, Niki Y, Totsuka K, Sunakawa K, Hanaki H, Hattori R, Terada M, Kozuki T, Maruo A, Morita K, Ogasawara K, Takahashi Y, Matsuda K, Hirose T, Miyao N, Hayashi T, Takeyama K, Kiyota H, Tomita M, Yusu H, Koide H, Kimura S, Yanaoka M, Sato H, Ito T, Deguchi T, Fujimoto Y, Komeda H, Asano Y, Takahashi Y, Ishihara S, Arakawa S, Nakano Y, Tanaka K, Fujisawa M, Matsui T, Fujii A, Yamamoto S, Nojima M, Higuchi Y, Ueda Y, Kanamaru S, Monden K, Tsushima T, Seno Y, Tsugawa M, Takenaka T, Hamasuna R, Fujimoto N, Sho T, Takahashi K, Inatomi H, Takahashi N, Ikei Y, Hayami H, Yamane T, Nakagawa M, Kariya S, Arima T. : The nationwide study of bacterial pathogens associated with urinary tract infections conducted by the Japanese Society of Chemotherapy. J Infect Chemother2011 Feb;17(1):126-38.
  5. Matsui H, Hanaki H, Inoue M, Akama H, Nakae T, Sunakawa K, Omura S. : Development of an Immunochromatographic Strip for Simple Detection of Penicillin Binding Protein 2'. Clin Vaccine Immunol. 2011 Feb;18(2):248-53.

2010

  1. Sakai F, Hanaki H, Barada K, Hirao Y, Inamatsu T, Nakae T, Sunakawa K. : A 25-year trace of methicillin-resistant Staphylococcus aureus dissemination in a geriatric hospital in Japan.　 Int J Gen Med. 2010 Dec 9;3:399-405.
  2. Sakai F, Hanaki H, Ikeda-Dantsuji Y, Hirao Y, Nonoyama M, Iwata S, Sato Y, Akita H, Nakae T, Sunakawa K. : Trends in empirical chemotherapy of bacterial meningitis in children aged more than 4 months in Japan: a survey from 1997 through 2008.　J Infect Chemother. 2010 Sep 29.
  3. Kobayashi Y, Ichioka M, Hirose T, Nagai K, Matsumoto A, Matsui H, Hanaki H, Masuma R, Takahashi Y,　Omura S, Sunazuka T. :Bottromycin derivatives: efficient chemical modifications of the ester moiety and evaluation of anti-MRSA and anti-VRE activities. Bioorg Med Chem Lett. 2010 Oct 15;20(20):6116-20.
  4. Ikeda-Dantsuji Y, Hanaki H, Sakai F, Tomono K, Takesue Y, Honda J, Nonomiya Y, Suwabe A, Nagura O, Yanagihara K, Mikamo H, Fukuchi K, Kaku M, Kohno S, Yanagisawa C, Nakae T, Yoshida K, Niki Y. : Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan. J Infect Chemother. 2011 Feb;17(1):45-51.
  5. Ikeda S, Hanaki H, Yanagisawa C, Ikeda-Dantsuji Y, Matsui H, Iwatsuki M, Shiomi K, Nakae T, Sunakawa K, Omura S. : Identification of the active component that induces vancomycin resistance in MRSA. J Antibiot (Tokyo)2010 Sep;63(9):533-8.
  6. Ishihara K, Shimokubo N, Sakagami A, Ueno H, Muramatsu Y, Kadosawa T, Yanagisawa C, Hanaki H, Nakajima C, Suzuki Y, Tamura Y. : Occurrence and molecular characteristics of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in an academic veterinary hospital. Appl Environ Microbiol2010 Aug;76(15):5165-74.
  7. Higuchi W, Mimura S, Kurosawa Y, Takano T, Iwao Y, Yabe S, Razvina O, Nishiyama A, Ikeda-Dantsuji Y, Sakai F, Hanaki H, Yamamoto T. : Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in a Japanese child, demonstrating multiple divergent strains in Japan. J Infect Chemother2010 Aug;16(4):292-7.
  8. Higuchi W, Hung WC, Takano T, Iwao Y, Ozaki K, Isobe H, Teng LJ, Shimazaki T, Honda A, Higashide M, Hanaki H, Yamamoto T. : Molecular characteristics of the Taiwanese multiple drug-resistant ST59 clone of Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus  from pediatric cellulitis.　 J Infect Chemother. 2010. Apr;16(2):144-9.